The Ryanodine Receptor (RyR) Carries its Own Counter-Ion Current in Rabbit Permeabilized Myocytes
نویسندگان
چکیده
منابع مشابه
Kinetics of FKBP12.6 binding to ryanodine receptors in permeabilized cardiac myocytes and effects on Ca sparks.
RATIONALE FK506-binding proteins FKBP12.6 and FKBP12 are associated with cardiac ryanodine receptors (RyR2), and cAMP-dependent protein kinase A (PKA)-dependent phosphorylation of RyR2 was proposed to interrupt FKBP12.6-RyR2 association and activate RyR2. However, the function of FKBP12.6/12 and role of PKA phosphorylation in cardiac myocytes are controversial. OBJECTIVE To directly measure i...
متن کاملOxidation of ryanodine receptor (RyR) and calmodulin enhance Ca release and pathologically alter, RyR structure and calmodulin affinity.
Oxidative stress may contribute to cardiac ryanodine receptor (RyR2) dysfunction in heart failure (HF) and arrhythmias. Altered RyR2 domain-domain interaction (domain unzipping) and calmodulin (CaM) binding affinity are allosterically coupled indices of RyR2 conformation. In HF RyR2 exhibits reduced CaM binding, increased domain unzipping and greater SR Ca leak, and dantrolene can reverse these...
متن کاملThree-dimensional distribution of ryanodine receptor clusters in cardiac myocytes.
The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat and ventricular atrial myocytes. The resolution of the three-dimensional structure was improved by a novel transverse sectioning meth...
متن کاملDeciphering ryanodine receptor array operation in cardiac myocytes
Elemental calcium signals from RYR arrays operating in cardiac myocytes have been extensively characterized with ever-improving optical methods and other innovative techniques. However, the exact nature of elemental calcium signals in terms of RYR gating in situ remains an enigma. Here, we synthesize insights gleaned from recent developments in single-channel resolution of cardiac RYR organizat...
متن کاملIon conduction and selectivity in the ryanodine receptor channel.
The ryanodine receptor channel is an intracellular membrane Ca2+-release channel. The investigation of ion translocation and discrimination in individual channels under voltage-clamp conditions has revealed that the channel can sustain very high rates of cation translocation, has high affinity for divalent cations and displays relatively poor discrimination between physiologically relevant cati...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.2976